Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. SpectraMax® M5e. Dagda Lab. 4) and cells were stained with 150 nM TSQ for 1 min. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. 3, La Jolla, CA, USA). The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. Roelli1, Amandine Stoss1, Rebecca A. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The percentage of viability of each treatment group in different. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 9000-0756. Measurement of Mitochondrial Membrane Potential by JC-1. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. All configurations. No. S. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Example: [Drawer]. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). SpectraMax® Paradigm® Multi-Mode Detection Platform. C. TEM. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. 03 in x 3. Serial number location on Series II and Multi-Mode plate readers. Thirty min later, each well was homogenized and the optical density (OD) was measured in a spectrometer (Spectramax M4, Molecular Devices) at 570 nm. 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. normalized response (Graphpad Prism 8. 6. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 4. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). Background-subtracted data values (mean +/- SD) are graphed. Table 2. Highly selective for dsDNA over ssDNA or RNA. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Our absorbance plate readers feature our PathCheck Sensor. All configurations. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Description 1. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). AquaMax® 2000. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The chloride and pH. 2. All configurations. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Italic and boldface type are used for emphasis. Serving Victoria's renovation and construction needs. . Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. VIEW DETAILS. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax® ABS Plus. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The final concentration of hemoglobin in feces was. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. To avoid injury, it is recommended that two. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Results were normalized and expressed as a percentage of live cells. com. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. When a feature or capability applies to only certain readers, this exception is noted. 10. Choose cables that meet the requirement described in this appendix. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The IC50 curves were calculated using variable slope for log (inhibitor) vs. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. All configurations. Allows you to use EOS lenses on a micro four thirds camera M4/3 K&F concept lens adapter. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. Table 2. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. The MTT reagent was incubated with cells for 4 h. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. STAKMAX. 3. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. 3, La Jolla, CA, USA). org) with optimized instrument settings (Table 1) were used to generate and analyze data. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 05, ** p <. Background-subtracted data values (mean +/- SD) are graphed. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 5 mg mL −1 with 0. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Because of possible losses from settling, particle data for the >7 µm size fraction (<0. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Cells were exposed to drug solutions with. normalized response (Graphpad Prism 8. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. Detection modalities are shown in Table 1-1. *Statistical significance: p < 0. FP assay do not perform well with protein:protein interactions. instruments you must first insert an adapter plate. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Cellular Reactive Oxygen Species (ROS) was measured in Neuro2a cells seeded in 6-well plate at a density of 5000 cells/well. Here, we present an approach to predict UV-visible absorption spectra of solvated aromatic molecules by quantum chemistry (QC) and machine learning (ML). The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Bioz Stars score: 86/100, based on 1 PubMed citations. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 This document is provided to customers who have purchased Molecular Devices,…spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485nm and emissionλ 535nm. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. Like all. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. 3. Images were visualized using a fluorescence microscope (Nikon, Japan) . The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. The IP-10 standard curve was prepared as described in Section 10. D 1 1. † Bottom mounting for SpectraMax Paradigm Readers only. Example: [Drawer]. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Roelli1, Amandine Stoss1, Rebecca A. Description 1. Microscopic evaluation of apoptosis. 0 statistics software. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. . Ltd. Qualify the luminescence performance of the SpectraMax . The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 5. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 4. MTT values at day 3 were normalized to day 0. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. ® reader to the computer. High tolerance for contaminants. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. After preparation, wrap in foil (see Note 1). The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. All experiments. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The chamber contains springs that position the cuvette in the proper alignment for a read. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 1. The following document is intended to demonstrate setup of this instrument. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. Published Dec 16, 2020 | Updated Dec 16, 2020. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 1. Results were expressed as a percentage relative to the control group (defined as 100%). INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. 2. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Spectramax M Series, supplied by Danaher Inc, used in various techniques. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). 1, BIO-RAD Laboratories). . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Three kits covering a wide range of dsDNA concentrations. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. TSQ was dissolved in Lock’s buffer (pH 7. . $2,219. 1step assay with SpectraMax microplate readers. ZERO BIAS - scores, article reviews, protocol conditions and moreNote: For the SpectraMax M2, M2e, M3, M4, M5, M5e, and FlexStation 3. SpectraMax® Paradigm® Multi-Mode Detection Platform. Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. ATP standard curves read on SpectraMax microplate readers. SpectraMax® M4). Plot the fluorescence intensity versus the cell seeding number. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. Each well was scanned at nine evenly distributed points and averaged automatically with the software. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. /600 em. The cleaved substrate was excited at 356-nm, and the fluorescence emissions were detected. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . Where is the instrument serial number located? The serial number is on. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. The available phosphorus in the soil was leached by double-acid extraction (0. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. 2 only) SpectraMax M-series Microplate readers. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Table 2. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Polybead® nanoparticles were analyzed. The SpectraMax M5e reader offers the additional benefit of being certified . 2. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 05 mg mL −1. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. WARNING! The instrument weighs approximately 36 pounds (16. Interpolated concentrations of native CRP in human serum and plasma samples. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 05 mg mL −1. , 2020b). The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. /525 em. Optimized for fluorescence microplate readers. SpectraMax Gemini XPS/EM Register or Sign In. 6 reviews. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Statistical. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. * BD Falcon® Microtest™ plates #353075 and # 353072). Dumont3, Matthias S. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Background-subtracted data values (mean +/- SD) are graphed. Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. SpectraMax M4 Multi-Mode Microplate Reader. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. In the M2, M5 and Plus. Optimized for fluorescence microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 315 can't find zero order. 4. All experiments were performed in triplicate. . 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. 2. org) with optimized instrument settings (Table 1) were used to generate and analyze data. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Note: In this user guide, all references to SpectraMax Multi-Mode. Recertification is recommended every two years. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. 2. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. STAKMAX. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Choose from a three- (M3), four- (M4), or Cables. After treatment, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, USA) was added to the PA and skatole treated hepatocytes and additionally incubated for 1 h. 304 signal level saturation. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. Background-subtracted data values (mean +/- SD, n =2) are graphed. Choose from a three- (M3), four- (M4), orCables. Detection modalities are shown in Table 1-1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. 1 & 7. 1 Measurement of Intestinal Permeability (In Vivo). SpectraMax M3, M4, M5, and M5 e. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax® M4. Lower limits of detection for ATP determined using the ATPlite . 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Examples: “Press carefully to engage,” “ Do not press down. 5 pM 2. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. SpectraMax® ABS. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). We therefore gathered salient. Results. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading 312 gain calibration failed. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. 5. Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. SpectraMax® M4). Five hours prior to when permeability will be measured, gavage mice with 0. After 7 days of culture, the colonies generated. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Table 1. The Multi-mode readers are top and bottom read. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. 90-0083 series): Excitation 530 nm,. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Data was calculated subtracting OD440 recorded with the final OD600 values. FlexStation™ III. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). Introduction. Turn the instrument around so that the back of the instrument is. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 38. TEM. 4) and cells were stained with 150 nM TSQ for 1 min. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). 305 reference level saturation. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. . 4 kilograms). 1. 2. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range.